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anti baiap3 antibody  (Proteintech)


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    Proteintech anti baiap3 antibody
    Identification of <t>BAIAP3</t> as a target of hsa-miR-1972 in LA. ( A ) The schematic diagram used to search for targets of hsa-miR-1972 in three databases of miRNA targets that were significantly up-regulated in WML tissues. A total of 32 targets of this miRNAs consistently predicted by three predictive tools showed up-regulation in WML tissues compared with non-lesional white matter samples obtained from subjects with WMLs. ( B ) Functional enrichment analysis of 32 targets of hsa-miR-1972 using DAVID software. Biological processes displaying a statistically significant change with P<0.05 are presented. ( C ) Expression of BAIAP3 in LA patients. The mRNA level of BAIAP3 was determined using real-time PCR in whole blood from type I LA subjects (n=12) and controls (n=12). ( D ) Diagram of predicted miR-1972 binding sites on the 3’UTR of BAIAP3 gene. The 3’UTR mutant sequence of BAIAP3 inserted in dual-luciferase reporter genes is indicated in blue. ( E ) Effect of hsa-miR-1972 on BAIAP3 mRNA transcription in 293T cells using the dual-luciferase reporter assay. Relative luciferase activity was analyzed in 293T cells co-transfected with pMRI-REPORT vector carrying either wild-type or mutated 3’UTR of BAIAP3 and hsa-miR-1972 mimic or relative negative control. ( F ) Effect of hsa-miR-1972 on the expression level of BAIAP3 protein in 293T cells observed in western blots. 293T cell lines were transfected with miR-1972 mimic (125 nM), miR-1972 inhibitor (125 nM), negative mimic control (125 nM), or negative inhibitor control (125 nM). Protein levels of BAIAP3 were detected at 48 h after post-transfection using western blotting. Tubulin was used as an internal control. ( G ) Quantitative analysis of results obtained from three independent experiments which are presented in ( F ). The results show that hsa-miR-1972 can significantly inhibit the expression of BAIAP3 in vitro . * P < 0.05, * P < 0.01, * P < 0.001.
    Anti Baiap3 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti baiap3 antibody/product/Proteintech
    Average 92 stars, based on 2 article reviews
    anti baiap3 antibody - by Bioz Stars, 2026-03
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    1) Product Images from "Integrated analysis of microRNA and mRNA expression profiling identifies BAIAP3 as a novel target of dysregulated hsa-miR-1972 in age-related white matter lesions"

    Article Title: Integrated analysis of microRNA and mRNA expression profiling identifies BAIAP3 as a novel target of dysregulated hsa-miR-1972 in age-related white matter lesions

    Journal: Aging (Albany NY)

    doi: 10.18632/aging.202562

    Identification of BAIAP3 as a target of hsa-miR-1972 in LA. ( A ) The schematic diagram used to search for targets of hsa-miR-1972 in three databases of miRNA targets that were significantly up-regulated in WML tissues. A total of 32 targets of this miRNAs consistently predicted by three predictive tools showed up-regulation in WML tissues compared with non-lesional white matter samples obtained from subjects with WMLs. ( B ) Functional enrichment analysis of 32 targets of hsa-miR-1972 using DAVID software. Biological processes displaying a statistically significant change with P<0.05 are presented. ( C ) Expression of BAIAP3 in LA patients. The mRNA level of BAIAP3 was determined using real-time PCR in whole blood from type I LA subjects (n=12) and controls (n=12). ( D ) Diagram of predicted miR-1972 binding sites on the 3’UTR of BAIAP3 gene. The 3’UTR mutant sequence of BAIAP3 inserted in dual-luciferase reporter genes is indicated in blue. ( E ) Effect of hsa-miR-1972 on BAIAP3 mRNA transcription in 293T cells using the dual-luciferase reporter assay. Relative luciferase activity was analyzed in 293T cells co-transfected with pMRI-REPORT vector carrying either wild-type or mutated 3’UTR of BAIAP3 and hsa-miR-1972 mimic or relative negative control. ( F ) Effect of hsa-miR-1972 on the expression level of BAIAP3 protein in 293T cells observed in western blots. 293T cell lines were transfected with miR-1972 mimic (125 nM), miR-1972 inhibitor (125 nM), negative mimic control (125 nM), or negative inhibitor control (125 nM). Protein levels of BAIAP3 were detected at 48 h after post-transfection using western blotting. Tubulin was used as an internal control. ( G ) Quantitative analysis of results obtained from three independent experiments which are presented in ( F ). The results show that hsa-miR-1972 can significantly inhibit the expression of BAIAP3 in vitro . * P < 0.05, * P < 0.01, * P < 0.001.
    Figure Legend Snippet: Identification of BAIAP3 as a target of hsa-miR-1972 in LA. ( A ) The schematic diagram used to search for targets of hsa-miR-1972 in three databases of miRNA targets that were significantly up-regulated in WML tissues. A total of 32 targets of this miRNAs consistently predicted by three predictive tools showed up-regulation in WML tissues compared with non-lesional white matter samples obtained from subjects with WMLs. ( B ) Functional enrichment analysis of 32 targets of hsa-miR-1972 using DAVID software. Biological processes displaying a statistically significant change with P<0.05 are presented. ( C ) Expression of BAIAP3 in LA patients. The mRNA level of BAIAP3 was determined using real-time PCR in whole blood from type I LA subjects (n=12) and controls (n=12). ( D ) Diagram of predicted miR-1972 binding sites on the 3’UTR of BAIAP3 gene. The 3’UTR mutant sequence of BAIAP3 inserted in dual-luciferase reporter genes is indicated in blue. ( E ) Effect of hsa-miR-1972 on BAIAP3 mRNA transcription in 293T cells using the dual-luciferase reporter assay. Relative luciferase activity was analyzed in 293T cells co-transfected with pMRI-REPORT vector carrying either wild-type or mutated 3’UTR of BAIAP3 and hsa-miR-1972 mimic or relative negative control. ( F ) Effect of hsa-miR-1972 on the expression level of BAIAP3 protein in 293T cells observed in western blots. 293T cell lines were transfected with miR-1972 mimic (125 nM), miR-1972 inhibitor (125 nM), negative mimic control (125 nM), or negative inhibitor control (125 nM). Protein levels of BAIAP3 were detected at 48 h after post-transfection using western blotting. Tubulin was used as an internal control. ( G ) Quantitative analysis of results obtained from three independent experiments which are presented in ( F ). The results show that hsa-miR-1972 can significantly inhibit the expression of BAIAP3 in vitro . * P < 0.05, * P < 0.01, * P < 0.001.

    Techniques Used: Functional Assay, Software, Expressing, Real-time Polymerase Chain Reaction, Binding Assay, Mutagenesis, Sequencing, Luciferase, Reporter Assay, Activity Assay, Transfection, Plasmid Preparation, Negative Control, Western Blot, Control, In Vitro



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    Identification of <t>BAIAP3</t> as a target of hsa-miR-1972 in LA. ( A ) The schematic diagram used to search for targets of hsa-miR-1972 in three databases of miRNA targets that were significantly up-regulated in WML tissues. A total of 32 targets of this miRNAs consistently predicted by three predictive tools showed up-regulation in WML tissues compared with non-lesional white matter samples obtained from subjects with WMLs. ( B ) Functional enrichment analysis of 32 targets of hsa-miR-1972 using DAVID software. Biological processes displaying a statistically significant change with P<0.05 are presented. ( C ) Expression of BAIAP3 in LA patients. The mRNA level of BAIAP3 was determined using real-time PCR in whole blood from type I LA subjects (n=12) and controls (n=12). ( D ) Diagram of predicted miR-1972 binding sites on the 3’UTR of BAIAP3 gene. The 3’UTR mutant sequence of BAIAP3 inserted in dual-luciferase reporter genes is indicated in blue. ( E ) Effect of hsa-miR-1972 on BAIAP3 mRNA transcription in 293T cells using the dual-luciferase reporter assay. Relative luciferase activity was analyzed in 293T cells co-transfected with pMRI-REPORT vector carrying either wild-type or mutated 3’UTR of BAIAP3 and hsa-miR-1972 mimic or relative negative control. ( F ) Effect of hsa-miR-1972 on the expression level of BAIAP3 protein in 293T cells observed in western blots. 293T cell lines were transfected with miR-1972 mimic (125 nM), miR-1972 inhibitor (125 nM), negative mimic control (125 nM), or negative inhibitor control (125 nM). Protein levels of BAIAP3 were detected at 48 h after post-transfection using western blotting. Tubulin was used as an internal control. ( G ) Quantitative analysis of results obtained from three independent experiments which are presented in ( F ). The results show that hsa-miR-1972 can significantly inhibit the expression of BAIAP3 in vitro . * P < 0.05, * P < 0.01, * P < 0.001.
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    Identification of <t>BAIAP3</t> as a target of hsa-miR-1972 in LA. ( A ) The schematic diagram used to search for targets of hsa-miR-1972 in three databases of miRNA targets that were significantly up-regulated in WML tissues. A total of 32 targets of this miRNAs consistently predicted by three predictive tools showed up-regulation in WML tissues compared with non-lesional white matter samples obtained from subjects with WMLs. ( B ) Functional enrichment analysis of 32 targets of hsa-miR-1972 using DAVID software. Biological processes displaying a statistically significant change with P<0.05 are presented. ( C ) Expression of BAIAP3 in LA patients. The mRNA level of BAIAP3 was determined using real-time PCR in whole blood from type I LA subjects (n=12) and controls (n=12). ( D ) Diagram of predicted miR-1972 binding sites on the 3’UTR of BAIAP3 gene. The 3’UTR mutant sequence of BAIAP3 inserted in dual-luciferase reporter genes is indicated in blue. ( E ) Effect of hsa-miR-1972 on BAIAP3 mRNA transcription in 293T cells using the dual-luciferase reporter assay. Relative luciferase activity was analyzed in 293T cells co-transfected with pMRI-REPORT vector carrying either wild-type or mutated 3’UTR of BAIAP3 and hsa-miR-1972 mimic or relative negative control. ( F ) Effect of hsa-miR-1972 on the expression level of BAIAP3 protein in 293T cells observed in western blots. 293T cell lines were transfected with miR-1972 mimic (125 nM), miR-1972 inhibitor (125 nM), negative mimic control (125 nM), or negative inhibitor control (125 nM). Protein levels of BAIAP3 were detected at 48 h after post-transfection using western blotting. Tubulin was used as an internal control. ( G ) Quantitative analysis of results obtained from three independent experiments which are presented in ( F ). The results show that hsa-miR-1972 can significantly inhibit the expression of BAIAP3 in vitro . * P < 0.05, * P < 0.01, * P < 0.001.
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    Identification of <t>BAIAP3</t> as a target of hsa-miR-1972 in LA. ( A ) The schematic diagram used to search for targets of hsa-miR-1972 in three databases of miRNA targets that were significantly up-regulated in WML tissues. A total of 32 targets of this miRNAs consistently predicted by three predictive tools showed up-regulation in WML tissues compared with non-lesional white matter samples obtained from subjects with WMLs. ( B ) Functional enrichment analysis of 32 targets of hsa-miR-1972 using DAVID software. Biological processes displaying a statistically significant change with P<0.05 are presented. ( C ) Expression of BAIAP3 in LA patients. The mRNA level of BAIAP3 was determined using real-time PCR in whole blood from type I LA subjects (n=12) and controls (n=12). ( D ) Diagram of predicted miR-1972 binding sites on the 3’UTR of BAIAP3 gene. The 3’UTR mutant sequence of BAIAP3 inserted in dual-luciferase reporter genes is indicated in blue. ( E ) Effect of hsa-miR-1972 on BAIAP3 mRNA transcription in 293T cells using the dual-luciferase reporter assay. Relative luciferase activity was analyzed in 293T cells co-transfected with pMRI-REPORT vector carrying either wild-type or mutated 3’UTR of BAIAP3 and hsa-miR-1972 mimic or relative negative control. ( F ) Effect of hsa-miR-1972 on the expression level of BAIAP3 protein in 293T cells observed in western blots. 293T cell lines were transfected with miR-1972 mimic (125 nM), miR-1972 inhibitor (125 nM), negative mimic control (125 nM), or negative inhibitor control (125 nM). Protein levels of BAIAP3 were detected at 48 h after post-transfection using western blotting. Tubulin was used as an internal control. ( G ) Quantitative analysis of results obtained from three independent experiments which are presented in ( F ). The results show that hsa-miR-1972 can significantly inhibit the expression of BAIAP3 in vitro . * P < 0.05, * P < 0.01, * P < 0.001.
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    Image Search Results


    Identification of BAIAP3 as a target of hsa-miR-1972 in LA. ( A ) The schematic diagram used to search for targets of hsa-miR-1972 in three databases of miRNA targets that were significantly up-regulated in WML tissues. A total of 32 targets of this miRNAs consistently predicted by three predictive tools showed up-regulation in WML tissues compared with non-lesional white matter samples obtained from subjects with WMLs. ( B ) Functional enrichment analysis of 32 targets of hsa-miR-1972 using DAVID software. Biological processes displaying a statistically significant change with P<0.05 are presented. ( C ) Expression of BAIAP3 in LA patients. The mRNA level of BAIAP3 was determined using real-time PCR in whole blood from type I LA subjects (n=12) and controls (n=12). ( D ) Diagram of predicted miR-1972 binding sites on the 3’UTR of BAIAP3 gene. The 3’UTR mutant sequence of BAIAP3 inserted in dual-luciferase reporter genes is indicated in blue. ( E ) Effect of hsa-miR-1972 on BAIAP3 mRNA transcription in 293T cells using the dual-luciferase reporter assay. Relative luciferase activity was analyzed in 293T cells co-transfected with pMRI-REPORT vector carrying either wild-type or mutated 3’UTR of BAIAP3 and hsa-miR-1972 mimic or relative negative control. ( F ) Effect of hsa-miR-1972 on the expression level of BAIAP3 protein in 293T cells observed in western blots. 293T cell lines were transfected with miR-1972 mimic (125 nM), miR-1972 inhibitor (125 nM), negative mimic control (125 nM), or negative inhibitor control (125 nM). Protein levels of BAIAP3 were detected at 48 h after post-transfection using western blotting. Tubulin was used as an internal control. ( G ) Quantitative analysis of results obtained from three independent experiments which are presented in ( F ). The results show that hsa-miR-1972 can significantly inhibit the expression of BAIAP3 in vitro . * P < 0.05, * P < 0.01, * P < 0.001.

    Journal: Aging (Albany NY)

    Article Title: Integrated analysis of microRNA and mRNA expression profiling identifies BAIAP3 as a novel target of dysregulated hsa-miR-1972 in age-related white matter lesions

    doi: 10.18632/aging.202562

    Figure Lengend Snippet: Identification of BAIAP3 as a target of hsa-miR-1972 in LA. ( A ) The schematic diagram used to search for targets of hsa-miR-1972 in three databases of miRNA targets that were significantly up-regulated in WML tissues. A total of 32 targets of this miRNAs consistently predicted by three predictive tools showed up-regulation in WML tissues compared with non-lesional white matter samples obtained from subjects with WMLs. ( B ) Functional enrichment analysis of 32 targets of hsa-miR-1972 using DAVID software. Biological processes displaying a statistically significant change with P<0.05 are presented. ( C ) Expression of BAIAP3 in LA patients. The mRNA level of BAIAP3 was determined using real-time PCR in whole blood from type I LA subjects (n=12) and controls (n=12). ( D ) Diagram of predicted miR-1972 binding sites on the 3’UTR of BAIAP3 gene. The 3’UTR mutant sequence of BAIAP3 inserted in dual-luciferase reporter genes is indicated in blue. ( E ) Effect of hsa-miR-1972 on BAIAP3 mRNA transcription in 293T cells using the dual-luciferase reporter assay. Relative luciferase activity was analyzed in 293T cells co-transfected with pMRI-REPORT vector carrying either wild-type or mutated 3’UTR of BAIAP3 and hsa-miR-1972 mimic or relative negative control. ( F ) Effect of hsa-miR-1972 on the expression level of BAIAP3 protein in 293T cells observed in western blots. 293T cell lines were transfected with miR-1972 mimic (125 nM), miR-1972 inhibitor (125 nM), negative mimic control (125 nM), or negative inhibitor control (125 nM). Protein levels of BAIAP3 were detected at 48 h after post-transfection using western blotting. Tubulin was used as an internal control. ( G ) Quantitative analysis of results obtained from three independent experiments which are presented in ( F ). The results show that hsa-miR-1972 can significantly inhibit the expression of BAIAP3 in vitro . * P < 0.05, * P < 0.01, * P < 0.001.

    Article Snippet: The membranes were then blocked overnight with 5% non-fat dried milk for 2 h and incubated with anti-BAIAP3 antibody (Cat. No: 24836-1-AP, Proteintech, USA) at 1:5000 dilution, and anti-Tubulin antibody (Cat. No: 11224-1-AP, Proteintech, USA) at 1:50,000 dilutions overnight at 4° C. After washing with TBST (10 mM Tris, pH 8.0, 150 mM NaCl, and 0.1% Tween20), the membranes were incubated for 4 h at room temperature with goat anti-rabbit second antibody at 1:20,000.

    Techniques: Functional Assay, Software, Expressing, Real-time Polymerase Chain Reaction, Binding Assay, Mutagenesis, Sequencing, Luciferase, Reporter Assay, Activity Assay, Transfection, Plasmid Preparation, Negative Control, Western Blot, Control, In Vitro